top of page
Elizabeth K. Strasko, Rebecca R. Piche, and Ben F. Brammell

Abstract.—The smallmouth salamander (Ambystoma texanum) is a widely distributed member of the family Ambystomatidae found throughout much of the central United States. We developed primers targeting a 147 base pair fragment of the mitochondrial cytochrome b region of the smallmouth salamander and tested these primers in silico, in vitro, and in vivo in laboratory-based studies. We believe these validated tools will be widely useful in the detection of A. texanum in breeding ponds, particularly in habitats where multiple species of Ambystoma occur.


Key Words.—conservation, eDNA, salamander, Ambystomatidae, Ambystoma texanum

Tennessee Journal of Herpetology, Vol. 6, pp. 4-17, 2023

ISSN 2576-9340


Strasko EK, RR Piche and BF Brammell. 2023. Development and validation of PCR primers for detecting Ambystoma texanum (Smallmouth Salamanders) from eDNA. Tennessee Journal of Herpetology. Vol. 6, pp. 4-17.

bottom of page